Endocrine Abstracts

In the ovary, 11βHSD enzymes inter-convert cortisol (F) with its inert metabolite, cortisone (E). Porcine follicular fluid from antral follicles and spontaneous ovarian cysts contains hydrophobic compounds that inhibit NADP(H)-dependent cortisol metabolism by 11βHSD1. The aim of this study was to measure intra-follicular F:E ratios in porcine antral follicles and ovarian cysts, and determine whether F:E ratios correlate with the inhibition of 11βHSD1 by porcine ...

In human GL cells, 11beta-HSD catalyses the interconversion of cortisol (F) with its inert 11-ketosteroid metabolite, cortisone (E). Since GL cells synthesise prostaglandins (PGs), and PGs have been shown to modulate the expression and activity of the cloned 11beta-HSD enzymes, the objective of the present study was to establish whether locally synthesised PGs affected the net oxidation of F to E by 11beta-HSD in human GL cells recovered from the ovaries of women undergoing co...

The enzymatic inactivation of cortisol is catalysed by isoforms of 11βHSD. 11βHSD1 is a bi-directional, NADP(H)-dependent enzyme whereas11βHSD2 is an NAD+-dependent dehydrogenase that inactivates cortisol. In the male rat, 11βHSD1 is highly expressed in the testis, epididymis, vas deferens and vesicular glands. Although 11βHSD activities have recently been reported in boar testicular tissue, cortisol metabolism in the boar reproductive tract...

In human granulosa-lutein (hGL) cells, cortisol (F) is inter-converted with cortisone (E) by 11BHSD. To date two isoforms of 11BHSD have been cloned. Human granulosa cells have been shown to express 11BHSD2 mRNA during the follicular phase of the ovarian cycle, switching to 11BHSD1 mRNA expression as luteinization occurs. However, it is not known whether (1) the protein expression, and (2) the 11BHSD enzyme activities reflect this pattern of mRNA expression. Hence, the aims of...